#SingleCell is an active hashtag on Bluesky. In the last 30 days, 18 people shared 49 posts with it — around 2 a day. Activity is up 117% versus the previous week, peaking on Jul 9 with 5 posts.

Posts with #SingleCell

Bart Deplancke

@bartdeplancke.bsky.social

10 months ago

🧵1/ Excited to share our new paper introducing a new #singlecell assay: scTF-seq, a high-throughput single-cell approach to explore how transcription factor (TF) dose shapes cell identity and reprogramming outcomes. 🔗 www.nature.com/articles/s41... Big congrats to the entire team @EPFL & @SIAT_China

www.nature.com

Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq - Nature Genetics

This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.

2 52 148

Conor McClune

@cmcclune.bsky.social

about 1 year ago

Today in Nature we report a systematic strategy to activate & identify gene sets in plants. We identify 8 new genes from the yew tree's Taxol biosynthetic pathway, enabling us to engineer tobacco with 17- & 20-gene pathways to Taxol precursors baccatin III & deBz-deoxy-Taxol #SingleCell #secmet 🧬🧪🌾

www.nature.com

Discovery of FoTO1 and Taxol genes enables biosynthesis of baccatin III - Nature

An approach that combines single-nucleus RNA sequencing and multiplexed perturbation identifies genes that enable the biosynthesis of direct precursors of the anti-cancer drug Taxol, whose curren...

5 45 120

Axel Visel

@axelvisel.bsky.social

about 1 year ago

No new genes needed to fly - just rewire what you have! 🦇🧬 Great new paper from the labs of @fany-real.bsky.social @stemundi.bsky.social @dariloops.bsky.social doi.org/10.1038/s415... #EvoDevo #SingleCell #BatWings

doi.org

Comparative single-cell analyses reveal evolutionary repurposing of a conserved gene programme in bat wing development - Nature Ecology & Evolution

Single-cell comparison of developing bat and mouse limbs reveals conservation of cell populations and gene expression patterns, and suggests repurposing of genes involved in proximal limb development ...

Max Planck Institute for Molecular Genetics @molgen.mpg.de

How did the bat get its wings? New research from a collaboration between the labs of Stefan Mundlos (MPIMG), Dario G. Lupiáñez, and Francisca M. Real (@cabd-upo-csic.bsky.social) show how genetic programs were reused during the evolution of mammalian flight. --> www.molgen.mpg.de/news-2025-fl...

www.molgen.mpg.de

3 44 118

Rebecca Berrens

@rberrens.bsky.social

about 1 year ago

Very happy to share our protocols paper for CELLO-seq. This will make single cell long read RNA-seq more accessible and provides analysis guidelines. We hope this helps the #transposon #TEsky community and folks working on #singleCell isoform and allelic #gene expression. doi.org/10.1038/s415...

doi.org

Long-read RNA sequencing of transposable elements from single cells using CELLO-seq - Nature Protocols

Single-cell long-read RNA sequencing enables the high-fidelity mapping of single-cell expression data from highly sequence-similar transposable elements to unique genomic loci by correcting errors fro...

8 38 106

Gioele La Manno

@gioelelamanno.bsky.social

over 1 year ago

Interested in #singlecell and #spatial #metabolomics? Then book your calendar for December 10th! – Our online, free event will summarize major achievements of the field in 2024. #metabolism #omics #MALDI #EPFL With Giovanni D'Angelo and our #PhD students we prepared for you a fantastic lineup. 🧵 1/5

2 33 65

Victor Javier

@vjsanchez.bsky.social

over 1 year ago

MCGAE: unraveling tumor invasion through integrated multimodal spatial transcriptomics #SingleCell 🧬🖥️ academic.oup.com/bib/arti….

academic.oup.com

MCGAE: unraveling tumor invasion through integrated multimodal spatial transcriptomics

Abstract. Spatially Resolved Transcriptomics (SRT) serves as a cornerstone in biomedical research, revealing the heterogeneity of tissue microenvironments.

1 11 63

Bart Deplancke

@bartdeplancke.bsky.social

over 1 year ago

Major milestone: Tabula Sapiens 2.0 maps tissue composition & TF expression in 175 cell types, identifying 745 ubiquitous & 890 cell type-specific TFs (many still uncharacterized -> #Codebook) w/ roles in tissue homeostasis, stress response & metabolism. #SingleCell www.biorxiv.org/content/1….

www.biorxiv.org

Tabula Sapiens reveals transcription factor expression, senescence effects, and sex-specific features in cell types from 28 human organs and tissues

The Tabula Sapiens is a reference human cell atlas containing single cell transcriptomic data from more than two dozen organs and tissues. Here we report Tabula Sapiens 2.0 which includes data from ni...

0 24 53

Natanella Illouz-Eliaz

@natanellae.bsky.social

3 months ago

Excited to share that I will be opening my lab this fall at the @weizmanninstitute.bsky.social in the Department of Plant & Environmental Sciences! I am looking for postdocs interested in plant biology, stress biology, and single-cell technologies. #PlantBiology #SingleCell #Epigenomics #Postdoc

10 22 53

Will Macnair

@willmacnair.bsky.social

over 1 year ago

Restarting a previous discussion on here: How convinced are #SingleCell people by cell-cell communication methods? I'm still at "not very" 😅 I see them used everywhere, but I don't know of any really good way to validate them... More importantly, I don't know of any *non-simulated* training data.

15 18 49

JesperOlsenLab

@jesperolsenlab.bsky.social

over 1 year ago

🚀 Excited to share our latest article in #singlecell proteomics published in Cell! We've developed SC-pSILAC to simultaneously measure protein turnover and abundance in single cells, unlocking the first large-scale, 2D proteomic insights at single-cell resolution! www.cell.com/cell/fulltex...

eur02.safelinks.protection.outlook.com

Global analysis of protein turnover dynamics in single cells

The SC-pSILAC method enables single-cell measurement of both protein abundance and turnover, providing notable advances in the depth and versatility of proteomic technologies.

2 12 46

Lena Boehme

@lenamboehme.bsky.social

over 1 year ago

Couldn't wish for a better BlueSky debut post: Our spatial atlas of the developing thymus is now published in Nature! 🥳 www.nature.com/articles/s41... Also check out the other papers in the new Human Cell Atlas collection: www.nature.com/collections/... #singlecell #spatial #multiomics

www.nature.com

A spatial human thymus cell atlas mapped to a continuous tissue axis - Nature

A quantitative morphological framework for the human thymus reveals the establishment of the lobular cytokine network, canonical thymocyte trajectories and thymic epithelial cell distributions in...

4 10 46

Dr. Jean Fan

@jef.works

over 1 year ago

We identify evidence of off-target probe binding in the 10x Genomics #Xenium v1 Human Breast Gene Expression Panel, compromising the accuracy of resulting #singlecell #spatialtranscriptomics profiles See our preprint for more details: www.biorxiv.org/content/1…. #AcademicSky 🧵👇 1/n

www.biorxiv.org

Evidence of off-target probe binding in the 10x Genomics Xenium v1 Human Breast Gene Expression Panel compromises accuracy of spatial transcriptomic profiling

The accuracy of spatial gene expression profiles generated by probe-based in situ spatially-resolved transcriptomic technologies depends on the specificity with which probes bind to their intended tar...

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